| Autor: |
Fitzpatrick KL; School of Earth and Environmental Sciences, University of Adelaide, Adelaide, Australia. kate.fitzpatrick@adelaide.edu.au, Reid RJ |
| Jazyk: |
angličtina |
| Zdroj: |
Plant, cell & environment [Plant Cell Environ] 2009 Oct; Vol. 32 (10), pp. 1357-65. Date of Electronic Publication: 2009 Jun 10. |
| DOI: |
10.1111/j.1365-3040.2009.02003.x |
| Abstrakt: |
Boron (B) enters cells as the uncharged boric acid, a small neutral molecule with sufficient lipid solubility to cross cell membranes without the aid of transport proteins. The extent to which the observed uptake rates for B in plants can be explained by this simple physical process was examined by applying treatments expected to inhibit the membrane transporters most likely to be involved in B transport. These experiments established that at least 50% of B uptake could be facilitated by transporters. The B transport characteristics of two barley aquaglyceroporins, HvPIP1;3 and HvPIP1;4, were investigated using yeast complementation assays. Expression of both genes in yeast resulted in increased B sensitivity. Transport assays in yeast confirmed that HvPIP1;3 and HvPIP1;4 are both capable of transporting B. The physiological role of these HvPIP1 genes in B transport is uncertain since their expression was not responsive to B nutritional status, and they continued to be expressed under toxicity conditions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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