[The construction and identification of the PRNP vectors with ubiquitin or the lysosome-targeting signal].
| Autor: | Li Y; Medical School, Anhui University of Science & Technology, Huainan 232001, China., Mei GY, Jiang HY, Wang GR, Tian C, Chen C, Wang X, Wang KX, Han J, Dong XP |
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| Jazyk: | čínština |
| Zdroj: | Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi] 2008 Dec; Vol. 22 (6), pp. 419-21. |
| Abstrakt: | Objective: To evaluate PrP expression characteristic of PRNP nucleic acid vaccine vector with ubiquitin or the lysosome-targeting signal. Methods: The gene of ubiquitin and lysosome-targeting signal were ligated to PRNP and pcDNA3.1 vector that is, pcDNA3.1-UPrP and pcDNA3.1-PrPL were constructed. The expression characteristics of PrP with two signals were evaluated by Western Blot and the localization was observed by indirect immune fluorescence. Results: The protein expressed by pcDNA3.1-UPrP and pcDNA3.1-PrPL with ubiquitin and lysosome-targeting signal can be recognized by prion-specific antibody. The protein has three glycosylation molecules form as native PrP.PrP with ubiquitin was degraded gradually with time extension,whereas quantity of PrP with lysosome signal reduced in 48 h after transfection. The protein with two location signals can direct fusion proteins to cytoplasm. Conclusion: The PRNP vectors with ubiquitin or the lysosome-targeting signal were constructed and expressed in eukaryocyte successfully. There will be one of good foundation on PRNP nucleic acid vaccine. |
| Databáze: | MEDLINE |
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