| Autor: |
Zagursky RJ; Du Pont Merck Pharmaceutical Company, Research and Development, Wilmington, DE 19880-0328., Conway PS, Kashdan MA |
| Jazyk: |
angličtina |
| Zdroj: |
BioTechniques [Biotechniques] 1991 Jul; Vol. 11 (1), pp. 36, 38. |
| Abstrakt: |
The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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