| Autor: |
Lièvre K; UMR Nancy-Université-INRA Agronomie et Environnement Nancy-Colmar, Vandoeuvre-Lès-Nancy, France., Tran TL, Doerper S, Hehn A, Lacoste P, Thomasset B, Bourgaud F, Gontier E |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2009; Vol. 547, pp. 235-48. |
| DOI: |
10.1007/978-1-60327-287-2_19 |
| Abstrakt: |
Agrobacterium tumefaciens is used to develop a genetic transformation method for a medicinal plant Ruta graveolens. The direct plant regeneration strategy is preferred to callus line establishment. In vitro seedlings, 2- -to 3-wk-old, are used to excise hypocotyls and co-cultivated for 3 d with A. tumefaciens strain C58C1Rif containing plasmid pTDE4 harbouring neomycin phosphotransferase (npt II, kanamycin resistance) and beta-glucuronidase encoding genes. The Southern blot analysis has shown that 78% kanamycin resistant plants contain gene encoding beta-glucuronidase. The GUS histochemical assay shows that 67% transgenic plants exhibit the corresponding enzymatic activity. Routine transformation efficiency of R. graveolens L. is 11% and could reach up to 22%. Transgenic plants are grown in the greenhouse within 4 months after the initial seedlings. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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