Effects of n-acetylcysteine on ischemic preconditioning: study in isolated rat hearts.
| Autor: | Oliveira DM; São Francisco de Assis Cardiovascular Foundation/ServCor - Belo Horizonte, MG, Brasil., Gomes ES, Mussivand T, Fiorelli AI, Gomes OM |
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| Jazyk: | English; Portuguese |
| Zdroj: | Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular [Rev Bras Cir Cardiovasc] 2009 Jan-Mar; Vol. 24 (1), pp. 23-30. |
| DOI: | 10.1590/s0102-76382009000100006 |
| Abstrakt: | Objective: The aim of this study is to assess if N-Acetylcysteine (NAC) changes the Ischemic Preconditioning (IP) in isolated rat hearts using only one cycle of IP. Methods: Heart Rate (HR), Coronary Flow (CF) and Myocardial Contractility (dP/dt) were registered in 30 Wistar rat's hearts. After anesthesia the hearts were removed and perfused with Krebes-Hensleit equilibrated solution with 95% of O2 and 5% of CO2 according Langendorff's method. GI: Control (n=6); GII: 20 min. ischemia (n=6); GIII: IP (n=6); GIV 50 microg/ml/min NAC before IP (n =6); GV: 100 microg/ ml/min NAC before IP (n=6). Parameters were measured after 15 min. of stabilization (T 0) and T3, T5, T10, T15, T20, T25 and T30 min. after reperfusion. Statistical significance was considered when P<0.05. Results: There were changes on HR comparing GI with GII at T20 and T25 and comparing GI with GIII, GIV with GV at T10 and T20 (P<0.05). CF was different comparing GI with GII at T3 and T5, GI with GIV at T10 and GI with GV at T10 and T25 (P<0.05). Myocardial Contractility was similar comparing GIII with GI and GV. GIII had higher dP/dt than GIV but without statistical difference (P>0.05). dP/dt was higher in GV than GIV but with statistically significant difference only at T30. Conclusion: dP/dt was better in preconditioned hearts and was changed if using NAC in GIV. The use of NAC didn't change the effects of preconditioning on myocardial contractility in GV. |
| Databáze: | MEDLINE |
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