| Autor: |
Mulvaney SP; Naval Research Laboratory, Washington, DC 20375-5342, USA. shawn.mulvaney@nrl.navy.mil, Ibe CN, Tamanaha CR, Whitman LJ |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 2009 Sep 15; Vol. 392 (2), pp. 139-44. Date of Electronic Publication: 2009 Jun 02. |
| DOI: |
10.1016/j.ab.2009.05.042 |
| Abstrakt: |
Herein, we describe the direct detection of genomic DNA using fluidic force discrimination (FFD) assays. Starting with extracted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in the presence of blocking and labeling sequences, and finally detected with microbead-based FFD assays. Both strain and species identification of extracted Bacillus DNA have been demonstrated in <30 min, without amplification (e.g., PCR). Femtomolar assays can be achieved with this rapid and simple procedure. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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