| Autor: |
Schudel BR; University of Illinois at Urbana-Champaign, Chemical & Biomolecular Engineering, Urbana, IL 61801, USA., Choi CJ, Cunningham BT, Kenis PJ |
| Jazyk: |
angličtina |
| Zdroj: |
Lab on a chip [Lab Chip] 2009 Jun 21; Vol. 9 (12), pp. 1676-80. Date of Electronic Publication: 2009 Apr 09. |
| DOI: |
10.1039/b901999e |
| Abstrakt: |
This paper reports the design, fabrication and validation of a microfluidic well plate for combinatorial screening applications. Each well within the array is comprised of two 200 picoliter compartments that each contain a photonic crystal biosensor to enable the on-chip, in situ detection of (bio-) molecular binding events. This microfluidic chip utilizes arrays of Actuate-to-Open valves to isolate all compartments, which allows the chip to be decoupled from pneumatic control lines and thus to be transported freely between filling, sensing and characterization platforms. A proof-of-principle 4 x 4 protein/antibody binding assay was performed to demonstrate the discrete mixing and on-chip sensing capabilities. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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