| Autor: |
Andriantsoanirina V; Malaria Unit Research, Institut Pasteur de Madagascar, Antananarivo, Madagascar., Lascombes V, Ratsimbasoa A, Bouchier C, Hoffman J, Tichit M, Rabarijaona LP, Durand R, Ménard D |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of microbiological methods [J Microbiol Methods] 2009 Aug; Vol. 78 (2), pp. 165-70. Date of Electronic Publication: 2009 May 22. |
| DOI: |
10.1016/j.mimet.2009.05.013 |
| Abstrakt: |
We have developed a High-Resolution DNA Melting method to detect mutations related to Plasmodium falciparum resistance. This method is based on real-time PCR followed by High Resolution Melting ramping from 67 degrees C to 80 degrees C with fluorescence data acquisition set at 0.1 degrees C increments. The accuracy of the technique was assessed using 177 P. falciparum clinical isolates and two reference strains. Results perfectly matched those obtained by DNA sequencing for some important genetic markers of P. falciparum resistance. This technique could be of great value for epidemiological studies, especially in developing countries. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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