| Autor: |
Tu Z; Department of Chemistry, Chemical Biology, and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA., Volk M, Shah K, Clerkin K, Liang JF |
| Jazyk: |
angličtina |
| Zdroj: |
Peptides [Peptides] 2009 Aug; Vol. 30 (8), pp. 1523-8. Date of Electronic Publication: 2009 May 21. |
| DOI: |
10.1016/j.peptides.2009.05.009 |
| Abstrakt: |
Many bioactive peptides are featured by their arginine and lysine rich contents. In this study, lysine and arginine residues in lytic peptides were selectively replaced by histidines. Although resulting histidine-containing lytic peptides had decreased activity, they did show pH-dependent cytotoxicity. The activity of the constructed histidine-containing lytic peptides increased 2-8 times as the solution pH changed from 7.4 to 5.5. More importantly, these histidine-containing peptides maintain the same cell killing mechanism as their parent peptides by causing cell lysis. Both the activity and pH-sensitivity of histidine-containing peptides are tunable by adjusting histidine substitution numbers and positions. This study has presented a general strategy to create bioactive peptides with desired pH-sensitivity to meet the needs of various applications such as cancer treatments. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect