| Autor: |
Doléans-Jordheim A; Université de Lyon, Lyon, France. anne.jordheim@recherche.univ-lyon1.fr, Cournoyer B, Bergeron E, Croizé J, Salord H, André J, Mazoyer MA, Renaud FN, Freney J |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2009 Sep; Vol. 28 (9), pp. 1105-11. Date of Electronic Publication: 2009 May 16. |
| DOI: |
10.1007/s10096-009-0755-z |
| Abstrakt: |
The purpose of this study was to evaluate the possibility of using a semi-automated repetitive DNA sequences-based polymerase chain reaction (rep-PCR) for typing Pseudomonas aeruginosa isolates. rep-PCR profiles obtained by the DiversiLab system of 84 P. aeruginosa isolates from distinct epidemiological situations were obtained. rep-PCR groupings were in good agreement with the origin of these isolates. Linked rep-PCR profiles were observed for isolates recovered from a same family of cystic fibrosis (CF) patients, for the etiological agents of clustered cases of nosocomial infections, and for some isolates recovered from a same hospital room. rep-PCR and pulsed-field gel electrophoresis SpeI restricted genomic DNA (PFGE-SpeI) profiles were compared. In a few instances, rep-PCR revealed genetic divergences among isolates of a same group of PFGE-SpeI profiles. These divergences could reflect genetic drifts among closely related isolates, as illustrated by those observed between clinical and environmental isolates of a same group of PFGE-SpeI profiles. The interpretation of such differences will require further studies, but the rep-PCR analysis of P. aeruginosa diversity appeared to be an appropriate method to investigate infra-specific genetic relatedness. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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