| Autor: |
Morlacchi P; Department of Chemistry, Rice University, Houston, Texas 77005, USA., Wilson WK, Xiong Q, Bhaduri A, Sttivend D, Kolesnikova MD, Matsuda SP |
| Jazyk: |
angličtina |
| Zdroj: |
Organic letters [Org Lett] 2009 Jun 18; Vol. 11 (12), pp. 2627-30. |
| DOI: |
10.1021/ol9005745 |
| Abstrakt: |
The triterpene product profile is reported for At5g36150 (PEN3), the last unexamined oxidosqualene cyclase in the reference plant Arabidopsis thaliana. PEN3 makes tirucalla-7,24-dien-3beta-ol ( approximately 85%) and several minor products. Also discussed are the unexpectedly facile convergent evolution of another Arabidopsis tirucalladienol synthase (LUP5), mechanistic origins of the 20S configuration, and active-site remodeling necessary to accommodate the 17alpha side chain. This work marks the first completed functional characterization of all triterpene synthases in a higher plant. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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