| Autor: |
Montanari J; Programa de Nanomedicinas, Laboratorio de Diseño de estrategias de Targeting de Drogas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, Buenos Aires, Argentina., Roncaglia DI, Lado LA, Morilla MJ, Romero EL |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of pharmaceutics [Int J Pharm] 2009 May 08; Vol. 372 (1-2), pp. 184-90. Date of Electronic Publication: 2009 Jan 21. |
| DOI: |
10.1016/j.ijpharm.2009.01.012 |
| Abstrakt: |
Although freeze-drying is an ordinarily used technique to dehydrate conventional liposomes, we have found that ultradeformable liposomes (UDLs) suffered irreversible aggregation when rehydrated upon freeze-drying (99.4% water elimination), even in high sugar content (4/1 sucrose/lipid mass ratio). When dehydrated by speed vac and vacuum drying, two alternative techniques that rendered less pronounced dehydration (94.27 and 96.2% water elimination, respectively) and avoid ice formation, however, UDL could only be successfully rehydrated when vacuum dried in 4/1 sucrose/lipid mass ratios. Conventional liposomes, on the other hand, were successfully reconstituted upon dehydrated by the three methods in lower sugar content (2/1 sucrose/lipid mass ratio). These results indicated that the 27% mole sodium cholate within the UDL lipid matrix was responsible for a greater and differential mechanical sensitivity of the bilayers to the different dehydration stress, as compared to conventional liposomes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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