| Autor: |
Lamberti Y; CINDEFI (UNLP, CONICET La Plata), School of Science, La Plata University, La Plata, Argentina., Alvarez Hayes J, Perez Vidakovics ML, Rodriguez ME |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS immunology and medical microbiology [FEMS Immunol Med Microbiol] 2009 Jul; Vol. 56 (2), pp. 143-50. Date of Electronic Publication: 2009 Apr 06. |
| DOI: |
10.1111/j.1574-695X.2009.00557.x |
| Abstrakt: |
Bordetella pertussis is a re-emerging human respiratory pathogen whose infectious process is not fully understood, hampering the design of effective vaccines. The nature of bacterial attachment to host cells is a key event in the outcome of the infection. However, host cell receptors involved in B. pertussis colonization of the respiratory tract are still under investigation. Here, we report that cholesterol-rich domains are involved in B. pertussis adhesion to epithelial cells. Treatment of A549 cells with cholesterol-sequestering drugs such as methyl-beta-cyclodextrin, nystatin, or filipin resulted in a significant decrease of B. pertussis attachment. Confocal laser microscopy studies showed B. pertussis associated with cholesterol-rich domains. Accordingly, B. pertussis was found in detergent-resistant membrane domain fractions isolated from bacterial-infected A549 cells. Our results indicate a main role of filamentous hemagglutinin, an environmentally regulated virulence factor, in this interaction, and a specific affinity for cholesterol, one of the major components of tracheal secretions, which might additionally contribute to the effective colonization of the respiratory tract. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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