| Autor: |
Wiśniewski JR; Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany. jwisniew@biochem.mpg.de, Zougman A, Nagaraj N, Mann M |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2009 May; Vol. 6 (5), pp. 359-62. Date of Electronic Publication: 2009 Apr 19. |
| DOI: |
10.1038/nmeth.1322 |
| Abstrakt: |
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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