| Autor: |
Vanderschuren H; Department of Biology, Institute of Plant Sciences, ETH Zürich, Zurich, Switzerland. hvanderschuren@ethz.ch, Alder A, Zhang P, Gruissem W |
| Jazyk: |
angličtina |
| Zdroj: |
Plant molecular biology [Plant Mol Biol] 2009 Jun; Vol. 70 (3), pp. 265-72. Date of Electronic Publication: 2009 Mar 20. |
| DOI: |
10.1007/s11103-009-9472-3 |
| Abstrakt: |
Cassava mosaic disease is a major constraint for cassava production in Africa, resulting in significant economic losses. We have engineered transgenic cassava with resistance to African cassava mosaic virus (ACMV), by expressing ACMV AC1-homologous hairpin double-strand RNAs. Transgenic cassava lines with high levels of AC1-homologous small RNAs have ACMV immunity with increasing viral load and different inoculation methods. We report a correlation between the expression of the AC1-homologous small RNAs and the ACMV resistance of the transgenic cassava lines. Characterization of the small RNAs revealed that only some of the hairpin-derived small RNAs fall into currently known small interfering RNA classes in plants. The method is scalable to stacking by targeting multiple virus isolates with additional hairpins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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