| Autor: |
Abo-Amer AE; Department of Biology, Faculty of Science, University of Taif, Saudi Arabia Kingdom. abo-amer@lycos.com, El-Deep BA, Altalhi AD |
| Jazyk: |
angličtina |
| Zdroj: |
Roumanian archives of microbiology and immunology [Roum Arch Microbiol Immunol] 2008 Jan-Jun; Vol. 67 (1-2), pp. 36-42. |
| Abstrakt: |
Growth of Lactobacillus plantarum AA135 in MRS broth at 30 degrees C yielded maximal plantaricin AA13 activity of 10000 AU/ml at pH 3.8 after 16 hours. Maximal activity (10000 AU/ml) of plantaricin AA13 was recorded in MRS broth at initial pH of 5 or 5.5. Optimal production (10000 AU/ml) was recorded in the presence of tryptone (20 g/l), a combination of tryptone plus meat extract (1:0.6) or tryptone with yeast extract (1:0.6) as sole nitrogen source. Growth of Lactobacillus plantarum AA135 in the presence of 20 g/l glucose yielded bacteriocin activity of 10000 AU/ml. Moreover, when 40 g/l glucose was added, the plantaricin AA13 levels doubled to 20000 AU/ml. Concentrations of 5, 10 and 20 g/l K2HPO4 lowered bacteriocin activity by 60%. Supplementing MRS with 1 g/l or more glycerol repressed bacteriocin production. Maximal activity of 20000 AU/ml was recorded in MRS supplemented with Vitamin DL-6,8-thioctic acid. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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