[Somatic mosaicism in mice detected using the RAPD-PCR method].

Autor: Butovskaia PR, Pavlova GV, Martirosian IA, Sukhikh GT, Ryskov AP
Jazyk: ruština
Zdroj: Molekuliarnaia genetika, mikrobiologiia i virusologiia [Mol Gen Mikrobiol Virusol] 2009 (1), pp. 3-7.
Abstrakt: 99 DNA samples of organs and tissues of 18 mice were examined using the method of PCR amplification with random primers. Among 27 oligonucleotide primers tested, 4 producing stable, well-reproducible profiles of amplification products were chosen for further analysis. Using 2 of these primers we detected differences in RAPD-profiles in some tissues in several individuals. These differences were associated with the modification of mobility, or with the fragment gain/loss in the RAPD profile, and could be caused either by genomic rearrangements, or mutations involving the regions of the DNA-primer pairing. Different epigenetic factors may also contribute to this process.
Databáze: MEDLINE