| Autor: |
Aberman LE, Petlychna LI, Lutsyk MD |
| Jazyk: |
ukrajinština |
| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1978) [Ukr Biokhim Zh (1978)] 1991 May-Jun; Vol. 63 (3), pp. 70-6. |
| Abstrakt: |
A method is developed to obtain lectin from jack fruit (Artocarpus integrifolia) seeds using an affinity chromatography on a sorbent prepared from the egg white. The minimum agglutination concentration of human erythrocytes is 80 ng/ml, the molecular weight of the preparation is about 39 kDa, it contains 1.8% of neutral hexoses and 3.1% of hexosamines. PAAG electrophoresis in the alkali system has revealed several molecular forms of lectin isolated by preparative electrophoresis, their properties are investigated. SDS-PAAG electrophoresis has revealed several types of polypeptide chains among which two chains (12 and 14 kDa) are predominant. Lectin possesses affinity to galactosides (not to free galactose) and N-acetylgalactosamine and interacts with O-glycans with high affinity. The preparation has mitogenic activity in optimal concentration 50 micrograms/ml. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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