| Autor: |
Rocha-González HI; Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados, Sede Sur, Calz. Tenorios 235, Deleg. Tlalpan, Col. Granjas Coapa, 14330 México, D.F., Mexico., Castañeda-Corral G, Araiza-Saldaña CI, Ambriz-Tututi M, Caram-Salas NL, Torres-López JE, Murbartián J, Granados-Soto V |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroscience [Neuroscience] 2009 Apr 21; Vol. 160 (1), pp. 156-64. Date of Electronic Publication: 2009 Feb 25. |
| DOI: |
10.1016/j.neuroscience.2009.02.033 |
| Abstrakt: |
mRNA and protein presence of Na+/H+ exchanger (NHE) 1 (NHE1) and 5 (NHE5) in dorsal root ganglion (DRG) and dorsal spinal cord as well as its possible role in three inflammatory nociception tests were determined. Local peripheral ipsilateral, but not contralateral, administration of NHE inhibitors 5-(N,N-dimethyl)amiloride hydrochloride (DMA, 0.3-30 microM/paw), 5-(N-ethyl-N-isopropyl)amiloride (EIPA, 0.3-30 microM/paw) and amiloride (0.1-10 microM/paw) significantly increased flinching but not licking behavior in the capsaicin and 5-HT tests. Moreover, DMA and EIPA (0.03-30 microM/paw) as well as amiloride (0.1-1 microM/paw) augmented, in a dose-dependent manner, 0.5% formalin-induced flinching behavior during phase II but not during phase I. Reverse transcription-polymerase chain reaction showed the expression of NHE1 and NHE5 in DRG and dorsal spinal cord. Western blot analysis confirmed the presence of NHE1 in DRG and spinal cord. Moreover, NHE5 was expressed in dorsal spinal cord, but not in DRG where a 45 kDa truncated isoform of NHE5 was identified. Collectively, these data suggest that NHE1, but not NHE5, plays an important role reducing inflammatory pain in rats. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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