Revisiting optical clearing with dimethyl sulfoxide (DMSO).

Autor: Bui AK; School of Biological Sciences, University of California, Irvine, California 92697, USA., McClure RA, Chang J, Stoianovici C, Hirshburg J, Yeh AT, Choi B
Jazyk: angličtina
Zdroj: Lasers in surgery and medicine [Lasers Surg Med] 2009 Feb; Vol. 41 (2), pp. 142-8.
DOI: 10.1002/lsm.20742
Abstrakt: Functional optical characterization of disease progression and response to therapy suffers from loss of spatial resolution and imaging depth due to scattering. Here we report on the ability of dimethyl sulfoxide (DMSO) alone to reduce the optical scattering of skin. We observed a threefold reduction in the scattering of skin with topical DMSO application. With an in vivo window chamber model, we observed a threefold increase in light transmittance through the preparation and enhanced visualization of subsurface microvasculature. Collectively, our data demonstrate the potential of DMSO alone to mitigate effects of scattering, which we expect will improve molecular imaging studies.
((c) 2009 Wiley-Liss, Inc.)
Databáze: MEDLINE
Externí odkaz: