| Autor: |
Reed J; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles Young Drive East, Los Angeles, California 90095, USA., Troke JJ, Schmit J, Han S, Teitell MA, Gimzewski JK |
| Jazyk: |
angličtina |
| Zdroj: |
ACS nano [ACS Nano] 2008 May; Vol. 2 (5), pp. 841-6. |
| DOI: |
10.1021/nn700303f |
| Abstrakt: |
Cancer and many other diseases are characterized by changes in cell morphology, motion, and mechanical rigidity. However, in live cell cytology, stimulus-induced morphologic changes typically take 10-30 min to detect. Here, we employ live-cell interferometry (LCI) to visualize the rapid response of a whole cell to mechanical stimulation, on a time scale of seconds, and we detect cytoskeletal remodeling behavior within 200 s. This behavior involved small, rapid changes in cell content and miniscule changes in shape; it would be difficult to detect with conventional or phase contrast microscopy alone and is beyond the dynamic capability of AFM. We demonstrate that LCI provides a rapid, quantitative reconstruction of the cell body with no labeling. This is an advantage over traditional microscopy and flow cytometry, which require cell surface tagging and/or destructive cell fixation for labeling. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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