| Autor: |
Ramanathan SP; BIOTEChnology Center Dresden, Dresden University of Technology, 01062 Dresden, Germany., van Aelst K, Sears A, Peakman LJ, Diffin FM, Szczelkun MD, Seidel R |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2009 Feb 10; Vol. 106 (6), pp. 1748-53. Date of Electronic Publication: 2009 Jan 30. |
| DOI: |
10.1073/pnas.0807193106 |
| Abstrakt: |
To cleave DNA, Type III restriction enzymes must communicate the relative orientation of two asymmetric recognition sites over hundreds of base pairs. The basis of this long-distance communication, for which ATP hydrolysis by their helicase domains is required, is poorly understood. Several conflicting DNA-looping mechanisms have been proposed, driven either by active DNA translocation or passive 3D diffusion. Using single-molecule DNA stretching in combination with bulk-solution assays, we provide evidence that looping is both highly unlikely and unnecessary, and that communication is strictly confined to a 1D route. Integrating our results with previous data, a simple communication scheme is concluded based on 1D diffusion along DNA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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