| Autor: |
Barns RJ, Elmslie RG |
| Jazyk: |
angličtina |
| Zdroj: |
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 1977 Aug 15; Vol. 79 (1), pp. 55-61. |
| DOI: |
10.1016/0009-8981(77)90460-0 |
| Abstrakt: |
A sensitive procedure is described for the determination in duodenal aspirates of enteropeptidase activity based on the activation of trypsinogen and the estimation of trypsin formed with benzoyl-arginine-p-nitroanilide. Using the recovery approach where a known amount of purified human enteropeptidase is diluted in duodenal fluid and the recoverable activity determined, this method was shown to give a sensitive and reliable estimate of the enteropeptidase activity in duodenal fluid although it was shown that the enzyme was subject to a 10% activation by components in the duodenal fluid. The reported 5-fold stimulation of enteropeptidase activity by bile salts could not be demonstrated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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