| Autor: |
Tomlinson ML; School of Biological Sciences, University of East Anglia, Norwich, UK., Guan P, Morris RJ, Fidock MD, Rejzek M, Garcia-Morales C, Field RA, Wheeler GN |
| Jazyk: |
angličtina |
| Zdroj: |
Chemistry & biology [Chem Biol] 2009 Jan 30; Vol. 16 (1), pp. 93-104. |
| DOI: |
10.1016/j.chembiol.2008.12.005 |
| Abstrakt: |
To dissect the function of matrix metalloproteinases (MMPs) involved in cellular migration in vivo, we undertook both a forward chemical genomic screen and a functional approach to discover modulators of melanophore (pigment cell) migration in Xenopus laevis. We identified the 8-quinolinol derivative NSC 84093 as affecting melanophore migration in the developing embryo and have shown it to act as a MMP inhibitor. Potential targets of NSC 84093 investigated include MMP-14 and MMP-2. MMP-14 is expressed in migrating neural crest cells from which melanophores are derived. MMP-2 is expressed at the relevant time of development and in a pattern that suggests it contributes to melanophore migration. Morpholino-mediated knockdown of both MMPs demonstrates they play a key role in melanophore migration and partially phenocopy the effect of NSC 84093. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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