| Autor: |
Essafi A; Cancer Research UK Laboratories, Department of Oncology, Imperial College London, London, UK., Gomes AR, Pomeranz KM, Zwolinska AK, Varshochi R, McGovern UB, Lam EW |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2009; Vol. 462, pp. 201-11. |
| DOI: |
10.1007/978-1-60327-115-8_13 |
| Abstrakt: |
This chapter describes methods for studying downstream events of the PI3K/Akt signaling cascade, focusing on the FoxO transcription factors. These approaches also represent alternative means for gauging the phosphoinositide-3 kinase/Akt activity. We describe protocols for the fractionation of cytoplasmic and nuclear protein extracts and for studying transcription factor DNA-binding activity in vitro and in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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