| Autor: |
Qian F; Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA. fqian_cn@hotmail.com, Aebig JA, Reiter K, Barnafo E, Zhang Y, Shimp RL Jr, Rausch KM, Jones DS, Zhu D, Lambert L, Mullen GE, Narum DL, Miller LH, Wu Y |
| Jazyk: |
angličtina |
| Zdroj: |
Microbes and infection [Microbes Infect] 2009 Mar; Vol. 11 (3), pp. 408-12. Date of Electronic Publication: 2008 Dec 27. |
| DOI: |
10.1016/j.micinf.2008.12.009 |
| Abstrakt: |
In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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