Evolution of regulatory sequences in 12 Drosophila species.
| Autor: | Kim J; Department of Computer Science, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America., He X, Sinha S |
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| Jazyk: | angličtina |
| Zdroj: | PLoS genetics [PLoS Genet] 2009 Jan; Vol. 5 (1), pp. e1000330. Date of Electronic Publication: 2009 Jan 09. |
| DOI: | 10.1371/journal.pgen.1000330 |
| Abstrakt: | Characterization of the evolutionary constraints acting on cis-regulatory sequences is crucial to comparative genomics and provides key insights on the evolution of organismal diversity. We study the relationships among orthologous cis-regulatory modules (CRMs) in 12 Drosophila species, especially with respect to the evolution of transcription factor binding sites, and report statistical evidence in favor of key evolutionary hypotheses. Binding sites are found to have position-specific substitution rates. However, the selective forces at different positions of a site do not act independently, and the evidence suggests that constraints on sites are often based on their exact binding affinities. Binding site loss is seen to conform to a molecular clock hypothesis. The rate of site loss is transcription factor-specific and depends on the strength of binding and, in some cases, the presence of other binding sites in close proximity. Our analysis is based on a novel computational method for aligning orthologous CRMs on a tree, which rigorously accounts for alignment uncertainties and exploits binding site predictions through a unified probabilistic framework. Finally, we report weak purifying selection on short deletions, providing important clues about overall spatial constraints on CRMs. Our results present a complex picture of regulatory sequence evolution, with substantial plasticity that depends on a number of factors. The insights gained in this study will help us to understand the combinatorial control of gene regulation and how it evolves. They will pave the way for theoretical models that are cognizant of the important determinants of regulatory sequence evolution and will be critical in genome-wide identification of non-coding sequences under purifying or positive selection. Competing Interests: The authors have declared that no competing interests exist. |
| Databáze: | MEDLINE |
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