| Autor: |
Mendoza-Rodríguez CA; Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, México, D.F., Mexico. adrimed@yahoo.com, Martínez MA, Vargas O, Nava K, Morimoto S, Espinosa M, Cerbón M |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular reproduction and development [Mol Reprod Dev] 2009 Jun; Vol. 76 (6), pp. 564-72. |
| DOI: |
10.1002/mrd.20988 |
| Abstrakt: |
Estrous cycle in mammals includes marked epithelial changes in reproductive tract, regulated by sex steroid hormones. In the present work we studied the activation of caspases and apoptotic pattern in uterine epithelial cells during proestrus and estrus, and the effect of mating in this process. In addition, we investigated the role of seminal vesicle secretions on apoptosis of uterine epithelia. Apoptotic index was evaluated by TUNEL assay, caspases-8, -9, and -3 activation was detected by Western blot and active caspase-3 expression was detected by immunohistochemistry. Our results show that mating during proestrus and estrus transition induced changes in the apoptotic pattern of uterine luminal epithelium during estrus, characterized by a delay in the onset of apoptosis as compared with that observed in nonmated rats. No differences in the apoptotic pattern in the glandular epithelium between mated and nonmated rats were observed. Seminal vesicle secretions inhibited luminal epithelium apoptosis, while no changes in glandular epithelium apoptosis were observed. We also demonstrate that activation of caspases-8, -9, and -3 occurred in both mated and nonmated rats. Active caspase-3 was detected in the luminal and glandular epithelium in both nonmated and mated rats. The overall results indicate that mating delays but does not prevent the cellular death of the rat uterine luminal epithelium and seminal vesicle secretions are involved in this delay. Finally, the activation of both the mitochondrial and the membrane receptor pathways of cell death are implicated in the molecular mechanism of uterine apoptosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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