| Autor: |
Douay L; Formation associée Claude Bernard sur les Greffes de cellules souches hématopoiétiques, CHU Saint-Antoine, Paris, France., Giarratana MC, Drouet X, Bardinet D, Gorin NC |
| Jazyk: |
angličtina |
| Zdroj: |
British journal of haematology [Br J Haematol] 1991 Sep; Vol. 79 (1), pp. 27-32. |
| DOI: |
10.1111/j.1365-2141.1991.tb08002.x |
| Abstrakt: |
We have previously reported prolonged in vitro maintenance of human bone marrow progenitor cells using a serum-free (SF) liquid culture system. The present study was undertaken to determine recombinant growth factor (rGF) requirement of long-term marrow culture (LTMC) in absence of exogenous serum, to avoid interference of any undefined components. Our data clearly show that the presence of serum is a major obstacle to the correct evaluation of rGF activity. However, in SF conditions the sequential analysis of these rGFs, alone or in combination, clearly showed a stimulating activity of interleukin 3 (IL3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and erythropoietin (EPO) on granulopoiesis and erythropoiesis. Indeed, the cumulative number of progenitors recovered during an 8-week period exceeded the initial input by a factor of 1.7 for granulocyte-macrophage (CFU-GM), of 3 for erythroid blast-forming units (BFU-E) and of 5.45 for CFU-E when EPO, GM-CSF and IL3 were combined. This study has confirmed that the system is able to sustain haematopoiesis for 8 weeks in a way similar to that in serum-dependent LTMC, despite diminished stromal adherent layer development which never covered more than 50% of the flask surface. We conclude that this defined SF-LTMC system provides a reproducible technique for studying human haematopoiesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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