[RNA interference silencing excision repair cross-complementing 1 gene and affecting cisplatin sensitivity on lung cancer cell lines].

Autor:	Shang XB; Department of Esophageal Tumor, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. sxbin@eyou.com, Yu ZT, Tang P, Zhang XZ
Jazyk:	čínština
Zdroj:	Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2008 Oct 28; Vol. 88 (39), pp. 2799-802.
Abstrakt:	Objective: To research the changes of cisplatin sensitivity by RNA interfering the excision repair cross-complementing (ERCC)1 gene in lung cancer cell lines. Methods: The small interference RNA (siRNA) targeting ERCC1 gene was designed and synthesized by transcription in vitro, and transfected to lung cancer cell line A549. The mRNA and protein of ERCC1 were evaluated by means of RT-PCR, Western blot and immunocytochemistry. The changes of cisplatin sensitivity after interference were examined by methyl thiazolyl tetrazolium (MTT) assay. Results: In A549 cell, the mRNA and protein levels of ERCC1 were dramatically decreased 24, 48 and 72 hours after transfection. The sensitivity to cisplatin of A549 cell line was increased by 3.07 times after disturbing the ERCC1 gene. Conclusions: The sensitivity to cisplatin of lung cancer cell lines A549 could be enhanced by RNA interfering ERCC1 gene.
Databáze:	MEDLINE
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