[Effect of microRNA143 expression on cell proliferation in colonic carcinoma].

Autor:	Liu H; Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China., Zhang SZ, Cai SR, Peng JP, Zheng S
Jazyk:	čínština
Zdroj:	Zhonghua zhong liu za zhi [Chinese journal of oncology] [Zhonghua Zhong Liu Za Zhi] 2008 Jul; Vol. 30 (7), pp. 498-501.
Abstrakt:	Objective: To investigate the effect of microRNA143 on cell proliferation and K-ras expression in colorectal carcinoma. Methods: Northern blot was used to examine the expression of miR-143 in colorectal carcinoma and adjacent normal tissues. A miR-143 expression vector was constructed and transfected into a human colon adenocarcinoma cell line SW480. Cell proliferation was evaluated by MTT assay. RT-PCR and Western blot were used to examine the expression of K-ras oncogene in transfected cells. Results: The level of mature miR-143 was lower in tumors compared with adjacent normal tissues in 81% of colorectal carcinoma specimens. In transfected cells, the increased accumulation of miR-143 inhibited the cell proliferation, and resulted in approximately 40.3% decrease of K-ras protein levels, but had no effect on level of K-ras mRNA. Conclusion: The increased accumulation of miR-143 inhibits the proliferation of transfected cells, and results in down-regulation of K-ras protein in colorectal carcinoma.
Databáze:	MEDLINE
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