The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration.

Autor: Jones BV; Institute for Animal Health, Compton, Newbury, Berkshire, UK., Hennion RM
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2008; Vol. 454, pp. 103-7.
DOI: 10.1007/978-1-59745-181-9_9
Abstrakt: Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
Databáze: MEDLINE