| Autor: |
De Pauw PE; Diabetes Research Center, Brussels Free University, B-1090 Brussels, Belgium. Pieter.Depauw@uzbrussel.be, Mackin RB, Goubert P, Van Schravendijk C, Gorus FK |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2009 Aug 01; Vol. 877 (23), pp. 2403-6. Date of Electronic Publication: 2008 Nov 19. |
| DOI: |
10.1016/j.jchromb.2008.11.024 |
| Abstrakt: |
We applied total error profiling to evaluate the conversion of a known proinsulin (PI) enzyme-linked immunosorbent assay (ELISA) into a time-resolved fluorescence immunoassay (TRFIA). The formula and acceptance criteria proposed by the Ligand Binding Assay Bioanalytical Focus Group (LBABFG) of the American Association of Pharmaceutical Scientists (AAPS) were applied. We found that the expected dynamic range enlargement with TRFIA compared to ELISA ([0.5-240] versus resp. [0.7-98] pmol/L) is limited by an interference of C-peptide when present in the sample at high concentrations (>7000 pmol/L). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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