Formation and maturation of parallel fiber-Purkinje cell synapses in the Staggerer cerebellum ex vivo.

Autor: Janmaat S; Department of Cell Biology, Molecular Imaging and Electron Microscopy, University Medical Center Groningen, University of Groningen, The Netherlands., Frédéric F, Sjollema K, Luiten P, Mariani J, van der Want J
Jazyk: angličtina
Zdroj: The Journal of comparative neurology [J Comp Neurol] 2009 Feb 01; Vol. 512 (4), pp. 467-77.
DOI: 10.1002/cne.21910
Abstrakt: In vivo, homozygous staggerer (Rora(sg/sg)) Purkinje cells (PCs) remain in an early stage of development with rudimentary spineless dendrites, associated with a lack of parallel fiber (PF) input and the persistence of multiple climbing fibers (CFs). In this immunocytochemical study we used cerebellar organotypic cultures to monitor the development of Rora(sg/sg) PF-PC synapses in the absence of CF innervation. Ex vivo the vesicular glutamate transporters VGluT1 and VGluT2 reactivity was preferentially localized around the Rora(sg/sg) PC soma and proximal dendrites, which are typically CF domains. The shift from VGluT2 to VGluT1 in PF terminals during development was delayed in Rora(sg/sg) slices. The postsynaptic receptors mGluR1 and GluRdelta2 were differently distributed on Rora(sg/sg) PCs. mGluR1 reactivity was evenly distributed in PC soma and dendrites, whereas GluRdelta2 reactivity, normally restricted at PF synapses, was dense in Rora(sg/sg) PC somata. The presynaptic distribution of VGluT1 and VGluT2 on Rora(sg/sg) PCs matched the postsynaptic distribution of the glutamate receptor GluRdelta2, but not mGluR1.
(2008 Wiley-Liss, Inc.)
Databáze: MEDLINE
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