Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics.

Autor: Oomen LC; Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. l.oomen@nki.nl, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K
Jazyk: angličtina
Zdroj: Journal of microscopy [J Microsc] 2008 Nov; Vol. 232 (2), pp. 353-61.
DOI: 10.1111/j.1365-2818.2008.02106.x
Abstrakt: The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23 degrees C counterparts.
Databáze: MEDLINE