| Autor: |
Darst SA; Department of Cell Biology, Stanford University School of Medicine, California 94305., Ahlers M, Meller PH, Kubalek EW, Blankenburg R, Ribi HO, Ringsdorf H, Kornberg RD |
| Jazyk: |
angličtina |
| Zdroj: |
Biophysical journal [Biophys J] 1991 Feb; Vol. 59 (2), pp. 387-96. |
| DOI: |
10.1016/S0006-3495(91)82232-9 |
| Abstrakt: |
Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free biotin-binding sites on the surface of the two-dimensional crystals facing the aqueous solution. The free biotin-binding sites could be specifically labeled with biotinylated ferritin. The streptavidin/biotinylated lipid system may provide a general approach for the formation of two-dimensional crystals of biotinylated macromolecules. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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