| Autor: |
Dongre C; Integrated Optical MicroSystems, MESA Institute for Nanotechnology, University of Twente, AE Enschede, The Netherlands. C.Dongre@ewi.utwente.nl, Dekker R, Hoekstra HJ, Pollnau M, Martinez-Vazquez R, Osellame R, Cerullo G, Ramponi R, van Weeghel R, Besselink GA, van den Vlekkert HH |
| Jazyk: |
angličtina |
| Zdroj: |
Optics letters [Opt Lett] 2008 Nov 01; Vol. 33 (21), pp. 2503-5. |
| DOI: |
10.1364/ol.33.002503 |
| Abstrakt: |
Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 microm, enabling high-resolution monitoring of the passage of different types of fluorescent analytes when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. Furthermore, we demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled DNA molecules, during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low limit of detection, will enable monitoring of extremely small quantities with high spatial resolution. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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