| Autor: |
Rat'ko AA; Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa., Stefan RI, van Staden JK, Aboul-Enein HY |
| Jazyk: |
angličtina |
| Zdroj: |
Talanta [Talanta] 2004 Jun 17; Vol. 63 (3), pp. 515-9. |
| DOI: |
10.1016/j.talanta.2003.11.036 |
| Abstrakt: |
In order to determine the enantiopurity of l-carnitine three enantioselective, potentiometric membrane electrodes were proposed for the assay of l-carnitine. The electrodes were designed using macrocyclic glycopeptide antibiotics-vancomycin and teicoplanin. Acetonitrile was added to the teicoplanine to design a modified teicoplanine based electrode. The linear concentration ranges for the proposed enantioselective membrane electrodes were 10(-4) to 10(-2)moll(-1) for electrodes based on vancomycin and teicoplanin and 10(-5) to 10(-2)moll(-1) for electrode based on teicoplanin modified with acetonitrile. The slopes of the electrodes were 56.5mV per pl-carnitine; 54.5mV per pl-carnitine and 58.3mV per pl-carnitine for vancomycin-, teicoplanin- and teicoplanin modified with acetonitrile-based electrodes, respectively. The enantioselectivity was determined over d-carnitine. The proposed electrodes could be employed reliably for the assay of l-carnitine raw material and its pharmaceutical formulation, Carnilean((R)) capsules. The surfaces of the electrodes are stable and easily renewable by polishing on alumina paper. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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