| Autor: |
Fragoso RR; Embrapa, Cerrados, Planaltina, DF, Brazil., Lourenço IT, Batista JA, Oliveira-Neto OB, Silva MC, Rocha TL, Coutinho MV, Grossi-de-Sa MF |
| Jazyk: |
angličtina |
| Zdroj: |
Experimental parasitology [Exp Parasitol] 2009 Feb; Vol. 121 (2), pp. 115-23. Date of Electronic Publication: 2008 Oct 07. |
| DOI: |
10.1016/j.exppara.2008.09.017 |
| Abstrakt: |
Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second-stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502kDa. Protein sequence comparisons of Mi-asp1 with GenBank (DQ360827) sequences showed 59-71% identity with nematode-specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant-parasitic nematode control. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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