Autor: |
Galkiewicz JP; University of South Florida, College of Marine Science, 140 7th Ave. S., St. Petersburg, Florida 33701, USA., Kellogg CA |
Jazyk: |
angličtina |
Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2008 Dec; Vol. 74 (24), pp. 7828-31. Date of Electronic Publication: 2008 Oct 17. |
DOI: |
10.1128/AEM.01303-08 |
Abstrakt: |
PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem. |
Databáze: |
MEDLINE |
Externí odkaz: |
|