Analysis of pH-induced population oscillations of Saccharomyces cerevisiae and Escherichia coli using photon correlation spectroscopy.

Autor: Jepras RI; Division of Biotechnology, P.H.L.S. Centre for Applied Microbiology and Research, Porton Down, Wilts, U.K., Rarity J, Perkins EA, Clarke DJ, Carr RJ, Atkinson T
Jazyk: angličtina
Zdroj: Biochemical Society transactions [Biochem Soc Trans] 1991 Apr; Vol. 19 (2), pp. 513-4.
DOI: 10.1042/bst0190513
Abstrakt: Following a recent successful application of p.c.s. to liquid chromatography in the biotechnology industry, its usefulness as a contamination monitor in the fermentation industry was assessed. It was found that: (i) the intensity bias of the technique limits its uses to the detection of contaminants when they are larger than the host; (ii) the inherent heterogeneity of microbial cultures prevents the use of multiangle studies, and (iii) the large size of bacteria make the use of p.c.s. in flowing, on-line systems impractical.
Databáze: MEDLINE