Measuring the lipid content of live animals using cyclopropane gas.

Autor: Henen BT; Environmental Biology Division, University of California, Los Angeles 90024.
Jazyk: angličtina
Zdroj: The American journal of physiology [Am J Physiol] 1991 Sep; Vol. 261 (3 Pt 2), pp. R752-9.
DOI: 10.1152/ajpregu.1991.261.3.R752
Abstrakt: The cyclopropane technique was validated, and it accurately estimated the lipid mass of six pond turtles (Trachemys scripta). This technique provided more accurate estimates of lipid mass and lipid-free mass (by difference) than other nonfatal techniques. This technique was accurate despite the turtles' low lipid contents (0.71-3.5% of body mass), variable water contents (65-74% of body mass), and variable body temperatures (approximately 23-31 degrees C). A computer model of the cyclopropane technique revealed that an analytical error of 1% in variables measured (e.g., body water content and body temperature) may cause errors of less than 1-6% in lipid estimates.
Databáze: MEDLINE