| Autor: |
Fox MH; Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, FRG., Arndt-Jovin DJ, Jovin TM, Baumann PH, Robert-Nicoud M |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of cell science [J Cell Sci] 1991 Jun; Vol. 99 ( Pt 2), pp. 247-53. |
| DOI: |
10.1242/jcs.99.2.247 |
| Abstrakt: |
The temporal course of replication monitored by 2- or 5-min pulses of bromodeoxyuridine (BrdUrd) incorporation in synchronized 3T3 cells was mapped by high-resolution light microscopy employing a charge-coupled device (CCD) camera and a confocal laser scanning microscope (CLSM). The cells were labeled simultaneously with monoclonal antibodies directed against BrdUrd and nuclear lamin, and stained with the A+T-specific dye 4',6-diamidino-2-phenylindole (DAPI). Stereoscopic reconstructions of cells showing both the lamin and BrdUrd distributions demonstrate that DNA replication occurs at discrete sites in the nucleus, the locations of which progress through a programmed sequence during S phase. Replication begins in a small number of sites in the interior of the nucleus exclusive of the nuclear membrane and proceeds rapidly in early S phase to encompass a relatively large number of small, discrete sites located throughout the nucleus, with the exception of the condensed heterochromatic regions. Replication is primarily confined to the condensed heterochromatic regions in mid-to-late S phase, and to the nuclear periphery at the end of S phase. These distinctive patterns demonstrate a programmed control of replication sites in the spatial domain in differentiated cell nuclei. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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