| Autor: |
Hiemstra PS; Dept. of Rheumatology, University Hospital Leiden, The Netherlands., Kramps JA, de Vreede TM, Breedveld FC, Daha MR |
| Jazyk: |
angličtina |
| Zdroj: |
Immunobiology [Immunobiology] 1991 Mar; Vol. 182 (2), pp. 117-26. |
| DOI: |
10.1016/S0171-2985(11)80195-6 |
| Abstrakt: |
The role of elastase and proteinase inhibitors in polymorphonuclear leukocyte(PMN)-mediated injury to human umbilical cord venous endothelial cells (HUVEC) was investigated. Both purified human neutrophil elastase and PMN that were stimulated with serum-treated zymosan (STZ) induced detachment, but not lysis of HUVEC. PMN-, but not purified elastase-mediated detachment was enhanced by the presence of methionine, which indicates a role for reactive oxygen metabolites in PMN-mediated HUVEC detachment. Detachment of HUVEC could be inhibited by secretory leukocyte proteinase inhibitor or antileukoprotease (ALP), alpha 1-proteinase inhibitor (alpha 1-PI) and N-methoxy-succinyl-ala-ala-pro-val-chloromethyl ketone (CMK). At concentrations at which elastase-mediated detachment was maximally inhibited, ALP and CMK, but not alpha 1-PI, were also able to inhibit maximally PMN-mediated detachment. An explanation for this difference could be that the larger size of alpha 1-PI reduces the access of alpha 1-PI to the interface between the PMN and the HUVEC. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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