| Autor: |
Shin JB; Oregon Hearing Research Center and Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Pagana J, Gillespie PG |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2009; Vol. 493, pp. 241-55. |
| DOI: |
10.1007/978-1-59745-523-7_14 |
| Abstrakt: |
Purification of hair bundles from inner-ear organs allows biochemical analysis of bundle constituents, including proteins and lipids. We describe here the "twist-off" method of bundle isolation, where dissected inner-ear organs are embedded in agarose, then subjected to a mechanical disruption that shears off bundles and leaves them in agarose blocks. With care in the dissection and in clean-up of the isolated bundles, contamination from cell bodies can be kept to a minimum. Isolated bundles can be analyzed by a variety of techniques, including immunocytochemistry, SDS-PAGE, immunoblotting, and mass spectrometry. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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