| Autor: |
Gallant SR; Process Sciences Department, BioMarin Pharmaceutical Inc, Novato, CA, USA., Koppaka V, Zecherle N |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2008; Vol. 421, pp. 53-60. |
| DOI: |
10.1007/978-1-59745-582-4_4 |
| Abstrakt: |
Immunonaffinity chromatography is a powerful technique for rapid purification of proteins. In a single-step purification, it is possible to purify proteins for testing in model systems and for conducting enzyme kinetic studies. Because the immunoaffinity-purified proteins are typically > 90-95% pure, depending on the starting material, interference from remaining contaminants is rare. This method describes an immunoaffinity chromatography technique for purifying proteins from over-expression in mammalian cell culture. The immobilization of the monoclonal antibody or polyclonal antiserum is presented. Conditions for purifying up to milligram quantities of protein are given, including a representative chromatogram. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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