| Autor: |
Garénaux A; UMR-INRA 1014 SECALIM ENVN/ENITIAA, Ecole Nationale Vétérinaire de Nantes, Route de Gachet-La Chantrerie, BP 40706, 44307 Nantes cedex 03, France. amelie.garenaux@igmors.u-psud.fr, Guillou S, Ermel G, Wren B, Federighi M, Ritz M |
| Jazyk: |
angličtina |
| Zdroj: |
Research in microbiology [Res Microbiol] 2008 Nov-Dec; Vol. 159 (9-10), pp. 718-26. Date of Electronic Publication: 2008 Aug 20. |
| DOI: |
10.1016/j.resmic.2008.08.001 |
| Abstrakt: |
Campylobacter jejuni is a microaerophilic pathogen representing one of the major causes of bacterial enteritis in humans. The oxidative stress response after exposure to paraquat, a strong oxidising agent, was analysed by two-dimensional protein electrophoresis and Maldi-ToF mass spectrometry. Oxidative stress and redox-related proteins were overexpressed: FldA flavodoxin and a pyruvate-flavodoxin oxidoreductase encoded by cj1476c. No increase in SodB expression was observed. An additional quantitative RT-PCR analysis showed an increase in katA but not in sodB expression. However, the sodB mutant was very sensitive to paraquat, its basal expression level being essential for oxidative stress resistance. Proteins related to iron homeostasis (Cft and a non-haem iron protein encoded by cj0012c) and general stress response (FusA and MreB) were found overexpressed. Interestingly, a two-component regulator encoded by cj0355c was differentially expressed in the presence of paraquat and could play a role in induction of the C. jejuni oxidative stress response. Virulence factors (CadF, FlaA and a VacJ homolog encoded by cj1371) were also found overexpressed under oxidative stress conditions and a cj1371 mutant showed increased sensitivity to paraquat, suggesting that the Cj1371 periplasmic protein could play a role in C. jejuni oxidative stress resistance. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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