Optical filtering systems for wavelength selection in light microscopy.

Autor: Waggoner AS; Carnegie Mellon University, Pittsburgh, Pennsylvania, USA., Wachman ES, Farkas DL
Jazyk: angličtina
Zdroj: Current protocols in cytometry [Curr Protoc Cytom] 2001 May; Vol. Chapter 2, pp. Unit 2.4.
DOI: 10.1002/0471142956.cy0204s15
Abstrakt: Because of its sensitivity and specificity, fluorescence-based detection is one of the foremost methods for microscopic imaging of biological tissues and cells. Dramatic improvements in filter system design and implementation coupled with development of an ever-widening range of sensitive fluorescent dyes have made multicolor imaging a powerful tool for structural and functional analysis. This revised and expanded unit reviews some of the main principles and developments of optical filtering.
Databáze: MEDLINE