| Autor: |
Trede NS; Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA. nikolaus.trede@hci.utah.edu, Ota T, Kawasaki H, Paw BH, Katz T, Demarest B, Hutchinson S, Zhou Y, Hersey C, Zapata A, Amemiya CT, Zon LI |
| Jazyk: |
angličtina |
| Zdroj: |
Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2008 Sep; Vol. 237 (9), pp. 2575-84. |
| DOI: |
10.1002/dvdy.21683 |
| Abstrakt: |
Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish (Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole-mount in situ hybridization for the expression of rag-1. To assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, alpha-embryonic globin expression was simultaneously assayed for multilineage defects. In this report, we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T-cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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