| Autor: |
Hundley HA; Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112, USA., Krauchuk AA, Bass BL |
| Jazyk: |
angličtina |
| Zdroj: |
RNA (New York, N.Y.) [RNA] 2008 Oct; Vol. 14 (10), pp. 2050-60. Date of Electronic Publication: 2008 Aug 21. |
| DOI: |
10.1261/rna.1165008 |
| Abstrakt: |
Adenosine deaminases that act on RNA (ADARs) are editing enzymes that convert adenosine to inosine in double-stranded RNA (dsRNA). ADARs sometimes target codons so that a single mRNA yields multiple protein isoforms. However, ADARs most often target noncoding regions of mRNAs, such as untranslated regions (UTRs). To understand the function of extensive double-stranded 3' UTR structures, and the inosines within them, we monitored the fate of reporter and endogenous mRNAs that include structured 3' UTRs in wild-type Caenorhabditis elegans and in strains with mutations in the ADAR genes. In general, we saw little effect of editing on stability or translatability of mRNA, although in one case an ADR-1 dependent effect was observed. Importantly, whereas previous studies indicate that inosine-containing RNAs are retained in the nucleus, we show that both C. elegans and Homo sapiens mRNAs with edited, structured 3' UTRs are present on translating ribosomes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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